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KMID : 0356420050230010024
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Journal of Korean Andrology
2005 Volume.23 No. 1 p.24 ~ p.29
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Expression of Hypoxia-inducible Factor-1꺙 and Vascular Endothelial Growth Factor in Corpus Cavernosum of the Type 2 Diabetes Mellitus Rat
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Lee Won-Ki
Ko Kyoung-Tae
Lee Sang-Wook
Suh Chang-Duk
Kim Sung-Yong
Kim Ha-Young
Yang Dae-Yul
Kim Doo-Man
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Abstract
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Purpose: The expression of hypoxia-inducible factor-1α(HIF-1) and vascular endothelial growth factor(VEGF) has been known as important factor of vascular complications in diabetes mellitus. The penile tumescence has variable degree according to vascularity. The aims of this study were to investigate erectile function and cavernosal expression of HIF-1α and VEGF in Otsuka Long-Evans Tokushima Fatty(OLETF) rats, which develop NIDDM naturally.
Materials and Methods: Ten male OLETF rats and ten control male Long-Evans Tokushima Fatty(LETO) rats were included in this study. The development of diabetes mellitus for OLETF were defined by glucose tolerance test at 26 weeks. After 72 weeks, OLETF rats, LETO rats and ten twenty-week male Sprague-Dawley rats were studied. Intracavernosal pressure were measured after cavernous nerve stimulation. Immunohistochemical stain and Western blot analysis were done for HIF-1α and VEGF in corpus cavernosum.
Results: Cavernous nerve stimulation-induced mean intracavernosal pressure(mmHg) was significantly decreased in the OLETF group(21.6/6.7 mmHg) compared to LETO(32.8/16.0 mmHg) group and in the LETO group compared to Sprague-Dawley group(47.2/11.7 mmHg)(p<0.05). With Western blot analysis, HIF-1α and VEGF expressions were higher in the OLETF group compared to other groups. With immunohistochemical stain, HIF-1α and VEGF were mainly expressed in corpus cavernosum and endothelium.
Conclusions: We propose that increased HIF-1α and VEGF expressions in OLETF rat penile tissue can be found at microvascular injury in corpus cavernosum and are presumed to be associated with vasculogenic erectile dysfunction.
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KEYWORD
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Diabetes mellitus, Erectile dysfunction, Hypoxia-inducible factor 1, Vascular endothelial growth factor
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